Since PCR was introduced, DNA replication has incrementally gotten easier and faster. Now, a revolution has happened. Next-generation PCR heats and cools instantly — "ramp rates" no longer exist. Using one thermocycler, you can run more than ten plates per hour. The math adds up quickly — 8 hours of 10 plates in a 384-well format means more than 30,000 data points in a single day.
You barely have time to walk away from your thermocycler. Melting to annealing happens in less than one tenth of a second; the total reaction time can be as little as two minutes.
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