NEXTGENPCR has been shown to speed the PCR amplification of DNA. In this technical note, we use NEXTGENPCR in the amplification step of our Novallele assay for the G6PC gene. Using the advanced mode function of NEXTGENPCR, we were able to reduce the time for a 35 cycle amplification to only 4 minutes and 30 seconds.
NEXTGENPCR amplification profile (Advanced Mode)
We subsequently analyzed the product by agarose gel electrophoresis to verify the length and look at the yield of the amplification product. We then analyzed by high-resolution melting analysis and determined the results were as expected for the Novallele assay.
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